Technical Data

S6504-25B
Clone Type
Monoclonal
Host
Rabbit
Source
Human
Isotype
IgG
Clone Number
2N8
Grade
Supernatant
Applications
WB
Crossreactivity
Hu Mo
Accession #
NM_005417
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-Src family, phosphorylated (Tyr416)

Src (also known as pp60src) is a non receptor Tyrosine Kinase involved in signal transduction in many biological systems and implicated in the development of human tumors. There are two critical phosphorylation sites of tyrosine on Src, tyrosine 418 and tyrosine 529 (referring to human Src sequence). The tyrosine 418 is located in the catalytic domain and is one of the autophosphorylation sites. Full catalytic activity of Src requires phosphorylation of tyrosine 418. This region of Src is also highly conserved in all of the related Src-family kinases, and thus prior immunoprecipitation may be required to identify which Src family member is being activated.

Applications
Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution
Western Blot: 1:1000 detected phospho-c-Src in RIPA lysates from COS cells transfected with active Src Optimal dilutions to be determined by the researcher.
Positive Control
RIPA cell lysate
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide containing the sequence ...NE[pY]TA…, where pY corresponds to phosphotyrosine at position 416 of c-Src.
Form
Supplied as a liquid in PBS, 0.05% sodium azide.
Purity
Supernatant
Specificity
Recognizes vertebrates Rsk3 phosphorylated (Thr353/Thr356). Species Crossreactivity: mouse

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Src protein-tyrosine kinase structure and regulation, Roskoski, Robert (2004) Biochem. Biophys. Res. Commun., 324: 1155-64 (2004), 2. Molecular interdiction of Src-family kinase signaling in hematopoietic cells, Geahlen, Robert L, et al. (2004) Oncogene, 23: 8024-32 (2004), 3. Role of STATs as downstream signal transducers in Src family kinase-mediated tumorigenesis, Silva, Corinne M (2004) Oncogene, 23:8017-23 (2004); 4. Structure and regulation of Src family kinases, Boggon, Titus J and Eck, Michael J (2004) Oncogene, 23: 7918-27 (2004), 5. The interplay between Src family kinases and receptor tyrosine kinases, Bromann, Paul A, et al (2004) Oncogene, 23:7957-68 (2004)|
USBio References
No references available
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