Mouse Anti-SREBP-1 (Sterol Regulatory Element Binding Protein-1, SREBP1, ADD1, D630008H06, Sterol Regulatory Element Binding Transcription Factor 1, SREBF1)
SREBP-1 and-2 are transcription factors which participate in the control of cholesterol homeostasis. SREBP proteins are attached to the endoplasmic reticulum and nuclear envelope. They are proteolytically cleaved and activated in response to conditions of low cellular sterol. Upon activation of SREBP-1 or -2, an ~480-500aa (N-terminal cleavage fragment of these proteins) enters the nucleus, activating transcription of enzymes and other proteins required for cholesterol synthesis. SCA (SREBP-cleavage activity) and caspase-3 proteases cleave SREBPs. SREBP proteins containing point mutations at caspase-3 cleavage sites (Asp460 in SREBP-1 and Asp468 in SREBP-2) do not become cleaved following induction of apoptosis. This suggests that SREBPs may play some role in apoptotic processes.
Applications
Suitable for use in Western Blot. Other applications not tested.
Recommended Dilutions
Western Blot: 1-2ug/ml for 2hrs at RT. Optimal dilution determined by the researcher.
Positive Control
HeLa and IMR5 cells
Cellular Localization
Cytoplasmic and nuclear
MW of Antigen
125kD (precursor) 60-70kD (cleaved)
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Recombinant protein encoding N-terminal aa301-407 (the bHLH/leucine zipper domain) of human SREBP-1. Epitope: aa301-407. MW of Antigen: Precursor: 125kD; Cleaved: 60-70kD.|Cellular Localization: Cytoplasmic and nuclear.
Form
Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide.
Purity
Purified by Protein G affinity chromatography from ascites.
Specificity
Recognizes human SREBP-1. Species Crossreactivity: hamster.