Rabbit Anti-SREBP-1 (Sterol Regulatory Element Binding Protein-1, SREBP1, ADD1, D630008H06, Sterol Regulatory Element Binding Transcription Factor 1, SREBF1)
Steroids are a large group of complex tetracyclic lipids that consist of a 17-carbon-ring system. Examples are bile acids, sterols, various hormones and saponins. These hormones are powerful signal molecules that regulate a host of organismal functions.
Sterol regulatory element binding proteins (SREBPs) are membrane-bound transcription factors that control the metabolism of cholesterol and fatty acids in animal cells. Two SREBPs, designated SREBP-1 and SREBP-2, have been isolated and cloned from several mammalian species. Human SREBP-1 and -2 are ~ 50% identical in aa sequence. They share the tripartite structure, and they both have the capacity to activate the same genes. Although the two proteins can form heterodimers, this does not appear necessary for their function.
SREBP1: Rat-1133aa; human-1147aa; human-1134aa; ~121.6kD; Chromosome 11B2. Isoform SREBP-1C predominates in liver, adrenal gland, brain and adipose tissue, whereas isoform SREBP-1A predominates in spleen. Isoform SREBP-1A and isoform SREBP-1C are found in kidney, thymus, testis, muscle, jejunum, and ileum.
Applications
Suitable for use in ELISA. Western Blot, though not tested, may potentially be used as an application. Other applications not tested.
Recommended Dilution
ELISA: 1:100,000 (50-100ng control peptide/well) Western Blot: 1-10ug/ml (chemiluminescence) Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Synthetic peptide corresponding to 16aa of human SREBP1, C-terminal (KLH).
Form
Supplied as a lyophilized powder from PBS, 0.05% sodium azide.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human SREBP1, isoform A.
