Mouse Anti-SR, p20 (Non-snRNP Splicing Factor) (FITC)
SR proteins are essential splicing factors involved in the use of both constitutive and alternative exons. Most splicing factors are concentrated within the mammalian cell nucleus in 20–40 distinct domains called speckles. SRp20 has been shown to activate exon 4 splicing.
Applications
Suitable for use in Immunofluorescence, FLISA and Western Blot. Other applications not tested.
Recommended Dilutions
FLISA: 0.1-1ug/ml Western Blot: 0.5-1ug/ml Immunofluorescence: 5-10ug Optimal dilutions to be determined by the researcher.
Positive Controls
HeLa cell extracts and HeLa cell nuclear extracts
Storage and Stability
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
A synthetic peptide corresponding to aa84-104 of the human SRp20 protein
Form
Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with Fluorescein isothiocyanate (FITC).
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes human non-snRNP splicing factor SRp20 and does not crossreact with other SR protein family members. Reactivity is not dependent upon phosphorylation of the SRp20 protein.
