Technical Data

S6570-51
Clone Type
Monoclonal
Host
Mouse
Source
Human
Isotype
IgG1,k
Clone Number
3G271 (7B4)
Grade
Affinity Purified
Applications
E IF WB
Crossreactivity
Hu
Shipping Temp
Blue Ice
Storage Temp
-20°C
Mouse Anti-SR, p20 (Non-snRNP Splicing Factor)

SR proteins are essential splicing factors involved in the use of both constitutive and alternative exons. Most splicing factors are concentrated within the mammalian cell nucleus in 20–40 distinct domains called speckles. SRp20 has been shown to activate exon 4 splicing.

Applications
Suitable for use in Immunofluorescence, ELISA and Western Blot. Other applications not tested.
Recommended Dilutions
ELISA: 0.1-1ug/ml Western Blot: 0.5-1ug/ml Immunofluorescence: 5-10ug Optimal dilutions to be determined by the researcher.
Positive Controls
HeLa cell extracts and HeLa cell nuclear extracts
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
A synthetic peptide corresponding to aa84-104 of the human SRp20 protein
Form
Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes human non-snRNP splicing factor SRp20 and does not crossreact with other SR protein family members. Reactivity is not dependent upon phosphorylation of the SRp20 protein.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Neugebauer, K.M. and Roth, M.B. Distribution of pre-mRNA splicing factors at sites of RNA polymerase II transcription. Genes and Development 11(9):1148–1159, 1997. 2. Misteli T, Caceres J.F., Spector D.L. The dynamics of a pre-mRNA splicing factor in living cells. Nature 387(6632):523–527, 1997. 3. Jumaa H., Nielsen P.J. Regulation of SRp20 exon 4 splicing. Biochem Biophys Acta 1494(1–2):137–43, 2000.
USBio References
No references available
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