Rabbit Anti-SSX2 (Synovial Sarcoma X Breakpoint 2, Cancer/testis Antigen 5.2, CT5.2, CT5.2a, Protein SSX2, SSX2A, SSX2B, Tumor Antigen HOM-MEL-40)
Synovial sarcoma, X breakpoint 2, also known as SSX2, is a human gene. The product of this gene belongs to the family of highly homologous synovial sarcoma X (SSX) breakpoint proteins. SSX1, SSX2 nd SSX4 genes have been involved in the t (X;18) translocation characteristically found in all synovial sarcomas. The SSX1 and SSX2 genes encode closely related proteins (81% identity) of 188aa that are rich in charged amino acids. The N-terminal portion of each SSX protein exhibits homology to the Kruppel-associated box (KRAB), a transcriptional repressor domain previously found nly in Kruppel-type zinc finger proteins.
Applications
Suitable for use in Western Blot and Immunohistochemistry . Other applications not tested.
Recommended Dilutions
Western Blot: 0.1-0.5 ug/ml Immunohistochemistry (Paraffin): 0.5-1ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile dH20. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Synthetic peptide corresponding to aa157-184, NREAQEKEERRGTAHRWSSQNTHNIGRF at the C-terminal of human SSX2.
Form
Supplied as a lyophilized powder from PBS, pH 7.4, 5% BSA, 0.05% thimerosal, 0.05% sodium azide. Reconstitute with 200ul sterile ddH2O.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human SSX2.
