Mouse Anti-S-Tag Epitope Tag (KETAAAKFERQHMDS)
The recombinant protein over-expression systems with well-known epitope tags are powerful strategies in checking and purifying the proteins of interest, which are expressed in certain vectors containing the particular tagged fragments. The small tags are used to be cloned along with the target proteins, and the antibodies against those fusion-tags are used to monitor fusion protein expression and purification. S-Tag is an epitope tag composed of a 15aa peptide (KETAAAKFERQHMDS) derived from pancreatic ribonuclease A. This antibody can be used to check S-tagged expressed proteins.
Applications
Suitable for use in ELISA, Flow Cytometry and Protein Purification. Other applications not tested.
Recommended Dilutions
Flow Cytometry: 1:5-1:10; 10ul labels 10e6 cells in 100ul Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
S-tag recombinant protein.
Form
Supplied as a liquid in borate buffered saline, pH 8.0. No preservative added.
Purity
Purified by affinity chromatography.
Specificity
Recognizes S-Tag, a fusion peptide tag derived from pancreatic RNase A that is widely used in protein expression applications.
