Technical Data

S7969-05G
Clone Type
Monoclonal
Host
Mouse
Source
Human
Isotype
IgG1,k
Clone Number
STAT1-79
Grade
Affinity Purified
Applications
E WB
Crossreactivity
Hu Mo Rt
Shipping Temp
Blue Ice
Storage Temp
-20°C
Mouse Anti-STAT 1 (Signal Transducer and Activator of Transcription 1)

STATs (signal transducers and activators of transcription) were originally discovered as two proteins (STAT 1 and 2) involved in IFN-a and IFN-g signal transduction. Since their initial identification, 5 more STAT proteins have been discovered (STAT 3, 4, 5a, 5b and 6). STATs undergo tyrosine phosphorylations in response to growth factor or cytokine signaling (in some cases mediated by JAK kinases [Janus Kinases 1, 2 and 3]), resulting in dimerization and translocation of STAT proteins to the nucleus. Phosphorylation at serine residues in certain STATs (STAT 1a, 3, 4 and 5) has also been reported, and appears to be required for the maximal activation of these proteins. This antibody recognizes proteins of ~91 and ~84kD, identified as STAT 1a and b. STAT 1 participates in a signaling pathway which is initiated by IFN-g.

Applications
Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution
Western Blot: 1ug/ml Optimal dilutions to be determined by the researcher.
Positive Control
Human Jurkat and HeLa cells, mouse L929 cells and rat thymocytes.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Recombinant protein corresponding to human STAT1a expressed in E. coli.
Form
Supplied as a liquid in PBS, pH 7.2, 1% BSA, 0.09% sodium azide.
Purity
Purified by affinity chromatography.
Specificity
Recognizes human STAT1a. Species Crossreactivity: Mouse and rat. Other species were not tested.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Burysek, L., T. Syrovets, and T. Simmet (2002) The serine protease plasmin triggers expression of MCP-1 and CD40 in human primary monocytes via activation of p38 MAPK and Janus kinase (JAK)/STAT signaling pathways. J. Biol. Chem. 277(36):33509-33517. 2. Schindler, C. and J.E. Darnell Jr. (1995) Transcriptional responses to polypeptide ligands: the JAK-STAT pathway. Annu. Rev. Biochem. 64:621-651. 3. Ihle, J.N. (1996) STATs: signal transducers and activators of transcription. Cell 84(3):331-334. 4. Wen, Z. (1995) Maximal activation of transcription by Stat 1 and Stat 3 requires both tyrosine and serine phosphorylation. Cell 82(2):241-250.
USBio References
No references available
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