Technical Data

S7975-11
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
IP WB
Crossreactivity
Hu Mk Mo Rt
Gene ID
10735
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-Stromal Antigen 2 (SA2, SA-2, STAG2, Cohesin Subunit SA-2, SCC3 Homolog 2)

The cohesin complex consists of a heterodimer between SMC1 (SMC1A or B) and SMC3, bound by additional RAD21 and STAG proteins (STAG1, 2 or 3) (1,2). These proteins form a ring-like structure that mediates the cohesion of two sister chromatids after DNA replication in S phase (1,2). RAD21 and STAG2 are phosphorylated by Polo-like kinase (PLK) during prophase, which leads to the dissociation of cohesin complexes from the chromosome arms; however, cohesin remains bound to centromeres until anaphase (3,4). RAD21 is cleaved by separin/ESPL1 in anaphase, which leads to dissociation of the remaining cohesin from centromeres, enabling sister chromatids to segregate during mitosis (5). RAD21 is also cleaved by caspase-3 and caspase-7 during apoptosis, resulting in a 64kD carboxy-terminal cleavage product that translocates to the cytoplasm and may help to trigger apoptosis (6,7). In addition to mediating cohesion of sister chromatids, the cohesin complex plays important roles in gene regulation and DNA repair, as SMC1 and SMC3 are both phosphorylated by ATM and ATR kinases upon DNA damage (1,2).

Applications
Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested.
Recommended Dilution
Western Blot: 1:1000, incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Immunoprecipitation: 1:100 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Synthetic peptide corresponding to the human STAG2 protein. Uniprot Accession: Q8N3U4
Form
Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
Purity
Purified by Protein A and peptide affinity chromatography.
Specificity
Recognizes STAG2 protein. Does not crossreact with other STAG proteins, including STAG1 and STAG3. Species Crossreactivity: mouse, rat and monkey

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Peters, J.M. et al. (2008) Genes Dev 22, 3089-114, 2. Barbero, J.L. (2009) Cell Mol Life Sci 66, 2025-35, 3. Hoque, M.T. and Ishikawa, F. (2001) J Biol Chem 276, 5059-67, 4. Hauf, S. et al. (2005) PLoS Biol 3, e69, 5. Hauf, S. et al. (2001) Science 293, 1320-3, 6. Pati, D. et al. (2002) Mol Cell Biol 22, 8267-77, 7. Chen, F. et al. (2002) J Biol Chem 277, 16775-81.
USBio References
No references available
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