Technical Data

S7975-63
Clone Type
Polyclonal
Host
Rabbit
Source
Mouse
Isotype
IgG
Grade
Affinity Purified
Applications
FC IHC IP WB
Crossreactivity
Mo Rt
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-SDF-1b (Stromal Cell-derived Factor-b, Pre-B Cell Growth-stimulating Factor, PBSF)

SDF-1b (stromal cell-derived factor-b), also known as pre-B-cell growth-stimulating factor (PBSF), is a 74-amino acid CXC chemokine originally cloned from a bone marrow stromal cell line. Targeted deletion of SDF-1 gene resulted in defects of B-cell lymphopoiesis and bone marrow myelopoiesis in mice. SDF-1 has been shown to be chemotactic for lymphocytes. In addition, SDF-1 was recently reported to be a ligand for CXCR4 (LESTR/fusin), a co-receptor for HIV-1 entry into T cells. SDF-1 binding to CXCR4 inhibits HIV-1 entry.

Applications
Suitable for use in Western Blot, Immunoprecipitation, Flow Cytometry and Immunohistochemistry. Other applications not tested.
Recommended Dilutions
Western Blot: 1:1000 Immunoprecipitation: 1:50 Flow Cytometry: 1:100 Immunohistochemistry: 1:100 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Recombinant protein corresponding to mouse SDF-1b expressed in E. coli
Form
Supplied as a liquid, 0.05% sodium azide
Purity
Purified by Protein A affinity chromatography
Specificity
Recognizes mouse SDF-1b. Species Crossreactivity: rat

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Tashiro, K., et al., Science 261: 600-603 (1993). 2. Nagasawa, T., et al., Proc. Natl. Acad. Sci. USA 91: 2305-2309 (1994). 3. Nagasawa, T., et al., Nature 328: 635-638 (1996). 4. Bleul, C.C., et al., J. Exp. Med. 184: 1101-1109 (1996). 5. Bleul, C.C., et al., Nature 382: 829-833 (1996). 6. Oberlin, E., et al., Nature 382: 833-835 (1996). 7. Reissa, K., et al., Neuroscience 115(1): 295-305 (2002). 8. Renata, S., et al., Blood 106(7): 2259-2268 (2005).
USBio References
No references available
United States Biological | 4 Technology Way | Salem, MA 01970
Phone 800-520-3011 | Fax 978-594-8052 | Website www.usbio.net