Rabbit Anti-STYX (Serine/Threonine/Tyrosine-interacting Protein, Protein Tyrosine Phosphatase-like Protein) (FITC)
STYX belongs to the protein-tyrosine phosphatase family, Non-receptor class subfamily. It contains one tyrosine-protein phosphatase domain and is a probable pseudophosphatase. It has a Gly residue instead of a conserved Cys residue in the dsPTPase catalytic loop which renders it catalytically inactive as a phosphatase. The binding pocket is however sufficiently preserved to bind phosphorylated substrates, and may be protect them from phosphatases. STYX seems to play a role in spermiogenesis.
Applications
Suitable for use in Western Blot. Other applications not tested.
Recommended Dilutions
Western Blot: 1:1000-1:2000; Band is observed at ~25.5kD using STYX-transfected 293T lysate Optimal dilutions to be determined by the researcher.
Storage and Stability
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
Full length protein corresponding to aa1-223 of human STYX. (NP_660294.1)
Form
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein isothiocyanate (FITC).
Specificity
Recognizes human STYX.
