Goat Anti-SUMF1 (Sulfatase Modifying Factor 1, AAPA3037, FGE, MGC131853, MGC150436, C-alpha-Formylglycine-Generating Enzyme)
SUMF1 activates sulfatases by converting their active site residue cysteine to formylglycine.
Applications
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution
ELISA: 1:16,000 Western Blot: 0.5-2ug/ml detects ~37-40kDa band observed in Mouse Pancreas, Mouse Eye and Human Kidney lysates (calculated MW of 40.6kDa according to Human NP_877437.2 and 40.6kDa according to Mouse NP_666049.2). Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to C-ETLNPKGPPSGKDR from the internal region of human SUMF1 (NP_877437.2).
Form
Supplied as a liquid in Tris saline, 0.02% sodium azide, pH7.3, 0.5% BSA.
Purity
Purified by affinity chromatography.
Specificity
Recognizes human SUMF1. Species crossreactivity: mouse. Sequence Homology: rat, bovine, canine.
