S8026-22A
Clone Type
PolyclonalHost
RabbitSource
HumanIsotype
IgGGrade
PurifiedApplications
E IHC WBCrossreactivity
HuAccession #
NP_001002255Shipping Temp
Blue IceStorage Temp
-20°CRabbit Anti-SUMO4, wild type (M55) (Small Ubiquitin-related Modifier 4, Small Ubiquitin-like Protein 4, SUMO-4, SMT3H4)
SUMO4 is a member of the SUMO gene family. This family of small ubiquitin-related modifiers covalently modify target lysines in proteins and control the target proteins' subcellular localization, stability, or activity. Upon oxidative stress, SUMO4 conjugates to various anti-oxidant enzymes, chaperones, and stress defense proteins. This protein may also conjugate to NFKBIA, TFAP2A and FOS, negatively regulating their transcriptional activity, and to NR3C1, positively regulating its transcriptional activity. Covalent attachment to SUMO4 substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I. In contrast to SUMO1, SUMO2 and SUMO3, SUMO4 seems to be insensitive to sentrin-specific proteases due to the presence of Pro-90. This may impair processing to mature form and conjugation to substrates. SUMO4 is located in the cytoplasm and specifically modifies IKBA, leading to negative regulation of NF-kappa-B-dependent transcription of the IL12B gene. The M55V substitution has been associated with type I diabetes.
Applications
Suitable for use in ELISA, Western Blot, and Immunohistochemistry. Other applications not tested.
Recommended Dilution
ELISA: 1:1,000 Western Blot: 1:100-1:500 Immunohistochemistry: 1:50-1:100 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide CEPRGLS(M)KQIRFRFG selected from human SUMO4 (KLH).
Form
Supplied as a liquid in PBS, 0.09% sodium azide.
Purity
Purified by ammonium sulfate precipitation
Specificity
Recognizes human SUMO4.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
References
1. Park,Y., et al. Nat. Genet. 37(2), 112 (2005) 2. Guo,D., et al. Nat. Genet. 36(8), 837-841 (2004) 3. Bohren,K.M.,et al. J. Biol. Chem. 279(26), 27233-27238 (2004) 4. Yang, S.H., et al., Mol. Cell 13(4):611-617 (2004). 5. Bailey, D., et al., J. Biol. Chem. 279(1):692-703 (2004). 6. Ling, Y., et al., Nucleic Acids Res. 32(2):598-610 (2004). 7. Pountney, D.L., et al., Exp. Neurol. 184(1):436-446 (2003). 8. Ohshima, T., et al., J. Biol. Chem. 278(51):50833-50842 (2003).USBio References
No references available