Rabbit Anti-Superoxide Dismutase 3 (Superoxide Dismutase 3 Extracellular, SOD 3, SOD3, Extracellular Superoxide Dismutase [Cu Zn], Extracellular Superoxide Dismutase Precursor, EC SOD, MGC20077) (Not for Export EU)
SOD3 is a member of the superoxide dismutase protein family. SODs are antioxidant enzymes that catalyse the dismutation of two superoxide radicals into hydrogen peroxide and oxygen. SOD3 is thought to protect the brain, lungs and other tissues from oxidative stress. It is secreted into the extracellular space and forms a glycosylated homotetramer that is anchored to the extracellular matrix and cell surfaces through an interaction with heparan sulfate proteoglycan and collagen. A small percentage of SOD3 is cleaved near the C-terminus before secretion to generate circulating tetramers that do not interact with the extracellular matrix.
Applications
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilutions
Western Blot: 1:1000-1:4000 Immunohistochemistry (Frozen/Paraffin): 1:100-1:2000 Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Synthetic peptide corresponding to aa18-37, WTGEDSAEPNSDSAEWIRD, of human extracellular Superoxide Dismutase, conjugated to diphtheria toxin.
Form
Supplied as a lyophilized powder. Reconstitute with 100ul sterile ddH2O.
Specificity
Recognizes human extracellular superoxide dismutase. Stains prominently specific human airway (probably Clara cells) and alveolar type II cells and some tubular cells in the kidney.
