S8060-25B
Clone Type
PolyclonalHost
RabbitSource
RatIsotype
IgGGrade
Affinity PurifiedApplications
E IHC IP WBCrossreactivity
Bo Ca Ch Dr Gp Hm Hu Mk Mo Po Rb Rt Sh XeShipping Temp
Blue IceStorage Temp
-20°CRabbit Anti-Superoxide Dismutase, Mn (SOD Mn)
Superoxide dismutase (SOD) is an endogenously produced intracellular enzyme present in almost every cell in the body (3). It works by catalyzing the dismutation of the superoxide radical O2- to O2 and H2O2, which are then metabolized to H2O and O2 by catalase and glutathione peroxidase (2,5). In general, SODs play a major role in antioxidant defense mechanisms (4). There are two main types of SOD in mammalian cells. One form (SOD1) contains Cu and Zn ions as a homodimer and exists in the cytoplasm. The two subunits of 16kD each are linked by two cysteines forming an intra-subunit disulfide bridge (3). The second form (SOD2) is a manganese containing enzyme and resides in the mitochondrial matrix. It is a homotetramer of 80kD. The third form (SOD3 or EC-SOD) is like SOD1 in that it contains Cu and Zn ions, however it is distinct in that it is a homotetramer, with a mass of 30kD and it exists only in the extracellular space (7). SOD3 can also be distinguished by its heparin-binding capacity (1).
Applications
Suitable for use in Western Blot, Immunoprecipitation, ELISA and Immunohistochemistry. Other applications not tested.
Recommended Dilution
Western Blot: 0.5ug/ml was sufficient for detection of Mn SOD in 20ug of rat brain tissue. Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Form
Supplied as a liquid in PBS, pH 7.0, 0.09% sodium azide, 50% glycerol.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes rat Mn Superoxide Dismutase (SOD) at 25kD. Species Crossreactivity: Human, Mouse, Bovine, Canine, Chicken, Drosophila, Guinea Pig, Porcine, Hamster, Monkey, Rabbit, Sheep, Xenopus.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
References
1. Adachi, T., et al., Clin. Chim. Acta. 212: 89-102 (1992). 2. Barrister, J.V., et al., Crit. Rev. Biochem. 22: 111-180 (1987). 3. Furukawa, Y. & O’ Halloran, T., Antioxidants & Redox Signaling. 8: 847-867 (2006). 4. Gao, B., et al., Am. J. Physiol. Lung Cell Mol. Physiol. 284: L917-L925 (2003). 5. Hassan, H.M., Free Radical Biol. Med. 5: 377-385 (1988). 6. Kurobe, N. & Kato, K., Biomed. Rsch. 12: 97-103 (1991). 7. Wispe, J.R., et al., BBA. 994: 30-36 (1989). 8. Xiao-Hong, L., et al., Brain Res. 625: 29-37 (1993).USBio References
No references available