Mouse Anti-SUZ12 (Suppressor of Zeste 12 Protein Homolog, CHET9, JJAZF1, KIAA0160, LOC23512) (PE)
Applications
Suitable for use in FLISA and Western Blot. Other applications not tested.
Recommended Dilution
Western Blot: 1:1000 Lysates from HeLa cells were resolved by electrophoresis, transferred to PVDF and probed with S8995-01. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and chemiluminescence detection. Optimal dilutions to be determined by the researcher.
Recommended secondary antibodies
I1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo I1904-06H IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo
Recommended Positive Antigen Control
H1838: HeLa nuclear extract.
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
Recombinant protein corresponding to full length human SUZ12.
Form
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes human SUZ12 at 95kD.
