Technical Data

S9104-03A2
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
WB
Crossreactivity
Hu Mo Rt
Gene #
SYN1
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-Synapsin I, phosphorylated (Ser9) (Synapsin 1, Synapsin-1, SYN1, SYN-1, Synapsin-I, SYNI, SYN-I, Brain Protein 4.1, SYN1a, SYN1b, SYN-1a, SYN-1b)

Synapsin is a neuronal phosphoprotein that coats synaptic vesicles, binds to the cytoskeleton, and is thought to function in the regulation of neurotransmitter release. Synapsin exists as a homodimer and at the subcellular level is located at the cell junction and synapse. It interacts with CAPON and forms a ternary complex with NOS1. Synapsin is a substrate of at least four different protein kinases and it is likely that phosphorylation plays a role in its regulation in the nerve terminal. Defects in SYN1 cause X-linked recessive epilepsy associated with variable learning disabilities and behavior disorders.

Applications
Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution
Western Blot: 1:500-1:1000 Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Synthetic phosphopeptide from the N-terminus of human synapsin.
Form
Supplied as a lyophilized powder from PBS, 5% BSA, 0.05% Thimerosal, 0.05% sodium azide. Reconstitute with 100ul sterile dH2O.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human Synapsin (Phosphos-Ser9). Species Crossreactivity: mouse, rat.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1.Sinha, D., et,al. (2005) Am J Physiol Renal Physiol ; 288: F703 - F713, 2.Onofri, F., et,al. (2000) J. Biol. Chem ; 275: 29857. 3.Bonanomi, D., et,al. (2005) J. Neurosci; 25: 7299 - 7308, 4.Tokumitsu, H., et,al. (2005) J. Biol. Chem ; 280: 35108 - 35118.
USBio References
No references available
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