Technical Data

S9108-16
Clone Type
Monoclonal
Host
Mouse
Source
Human
Isotype
IgM
Clone Number
4E206
Grade
Purified
Applications
IHC WB
Crossreactivity
Bo Hu Mo Rt
Shipping Temp
Blue Ice
Storage Temp
-20°C
Mouse Anti-Synaptophysin

Synaptophysin is an ~38kD integral membrane protein involved in neurotransmitter exocytosis present on synaptic vesicles. This protein consists of four transmembrane domains, with its amino- and carboxyl-terminus facing the cytoplasm. Due to its transmembrane domain structure, synaptophysin has been postulated to be a gap junction-like protein because voltage-dependent channel activity is seen when synaptophysin hexamers are reconstituted into lipid membranes. Recent studies have shown synaptophysin to be a major cholesterol-binding protein in brain synaptic vesicles and it has been proposed that this cholesterol-binding ability may be of key importance in the generation of synaptic vesicles at plasma membranes. A regional reduction of synaptophysin was found in patients with Alzheimer’s disease and schizophrenia.

Applications
Suitable for use in Western Blot and Immunohistochemistry. Other applications have not been tested.
Recommended Dilutions
Western Blot (Colorimetric): 1:2000 Immunohistochemistry: 1:50 using human neuroblastoma tissue Optimal dilutions to be determined by researcher.
Positive Controls
Mouse and rat brain tissue extract
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synaptic vesicle-containing fractions immunoprecipitated from |human brain homogenates.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide, 50% glycerol.
Purity
Purified by ammonium sulfate precipitation
Specificity
Recognizes human synaptophysin at ~38kD. |Species Crossreactivity: mouse, rat, bovine. No reactivity was detected in mouse liver extracts.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Honer, W.G., Hu, L. and Davies, P., Brain Research 609: 9-20 (1993). 2. Rehm, H., Wiedenmann, B. and Betz, H., EMBO J. 5: 535-541 (1986). 3. Thomas, L., et al., Science 242: 1050-1053 (1988). 4. Thiele, C., et al., Nat. Cell Biol. 2: 42-49 (2000). 5. Martin, T.F., Nat. Cell Biol. 2: E9-E11 (2000). 6. Landen, M., et al., Biol. Psychiatry 46: 1698-1702 (1999). 7. Honer, W., et al., Neurobiol. Aging 13: 373-382 (1992).
USBio References
No references available
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