Rabbit Anti-Syndecan-1 (CD138, SDC, SYND1, Syndecan) (Not for Export EU)
Syndecan-1 is a transmembrane (Type I) heparan sulfate proteoglycan and is a member of the syndecan proteoglycan family. The syndecans mediate cell binding, cell signaling, and cytoskeletal organization. Bearing properties of both heparan sulfate and chondroitin sulfate, Syndecan-1 functions as a cell surface proteoglycan membrane protein and participates in cell proliferation, cell migration and cell-matrix interactions via its receptor for extracellular matrix proteins. Altered Syndecan-1 expression has been detected in several different tumor types.
Applications
Suitable for use in Western Blot. Other applications not tested.
Recommended Dilutions
Western Blot: 1:1000 using mouse liver lysate. Proteins were visualized using a IgG (HRP) Dk x Rb and chemiluminescence detection. A dimer of Syndecan-1 was identified at ~77kD while monomeric Syndecan-1 is visible at ~32kD. The mature form, with heparan and chondroitin sulfate addtions, can sometimes be seen between 500-1000kD but may need to be solubilized from whole tissues by urea extraction. Triton X-100 or other mild detergents are enough to release the immature precursor forms. Optimal dilutions to be determined by the researcher.
Recommended Secondary Antibodies
I1904-46E: IgG, H&L (HRP) Pab Dk x Rb I1904-46K: IgG, H&L, X-Adsorbed (HRP) Pab Dk x Rb I1903-89G5: IgG, H&L, X-Adsorbed (HRP) Pab Dk x Rb I1904-45P6: IgG, H&L (HRP) Pab Dk x Rb
Positive Control
Mouse liver lysate
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
His-tagged full length recombinant protein corresponding to Syndecan-1. Species Sequence Homology: human, 100%. |Cellular Localization: Membrane; Single-pass type I membrane protein.
Form
Supplied as a liquid, 0.05% sodium azide.
Specificity
Recognizes mouse Syndecan-1 protein.
