Technical Data

S9111-30C
Clone Type
Monoclonal
Host
Mouse
Source
Human
Isotype
IgG1
Clone Number
B-A38
Grade
Affinity Purified
Applications
FC IHC
Crossreactivity
Hu
Shipping Temp
Blue Ice
Storage Temp
-20°C
Mouse Anti-Syndecan (SDC, CD138, CD138 Antigen, Heparan Sulfate Proteoglycan Fibroblast Growth Factor Receptor, Syndecan 1, Syndecan-1, Syndecan-1 Precursor, SDC1, SDC-1, SYND1)
Applications
Suitable for use in Flow Cytometry and Immunohistochemistry (frozen and paraffin). Other applications not tested.
Recommended Dilution
Flow Cytometry: Neat; 10ul labels 10e6 cells in 100ul Immunohistochemistry (frozen): 1:100-1:500 Immunohistochemistry (paraffin): 1:100 Optimal dilutions to be determined by the researcher.
Hybridoma
X63 Ag8.653 myeloma cells with spleen cells from BALB/c (Iffa Credo) mice.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
U266 cell line.
Form
Supplied as liquid in PBS, 1% BSA, 0.09% sodium azide.
Purity
Purified by Protein G affinity chromatography.
Specificity
Recognizes human Syndecan-1 (CD138).

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Borset, M. et al. (1993) Lack of IL-1 secretion from human myeloma cells highly purified by immunomagnetic separation. Br. J. Haem. 85: 446, 2. Anttonen, A. et al. (1999) Syndecan-1 expression has prognostic significance in head and neck carcinoma. Brit. J. Cancer 79: 558 - 564.
USBio References
No references available
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