Technical Data

S9116-01
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Serum
Applications
IHC
Crossreactivity
Hu Rt
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-Synphilin 1 (Synphilin-1, SYPH1, Alpha Synuclein-interacting Protein, MGC39814, Synuclein alpha Interacting Protein (Synphilin), SNCAIP) (Not for Export EU)

Synuclein alpha interacting protein (Synphilin-1) contains several protein-protein interaction domains and interacts with alpha synuclein in neurons. Mutations of SNCAIP have been linked to Parkinson disease. The amino acid sequence of synphilin-1 shares a high level of identity with its human counterpart, particularly in regions containing ankyrin-like motifs and the coiled-coil domain. Expression pattern of synphilin-1 in tissues is similar in both mouse and human. Synphilin-1 has an important role in the formation of aggregates and cytotoxicity in Parkinson disease and also Dorfin may be involved in the pathogenic process by ubiquitylation of synphilin-1.

Applications
Suitable for use in Immunohistochemistry. Other applications not tested.
Recommended Dilution
Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
A synthetic peptide (SL ELNGEKDKDK GRTLQRT) as part of human synphilin-1a conjugated to KLH.
Form
Supplied as a lyophilized powder. Reconstitute in 100ul of sterile water. Centrifuge to remove any insoluble material.
Purity
Serum
Specificity
A high level of specificity has been shown for this antiserum by IHC on human brain. Species Crossreactivity: This antiserum is known to react with rat and human synphilin-1a.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Kruger,R. Cell Tissue Res. 318 (1), 195-199 (2004) ; 2. Lee,G., etal. J. Biol. Chem. 279 (8), 6834-6839 (2004) ; 3. Tanaka,M., et al. J. Biol. Chem. 279 (6), 4625-4631 (2004) ; 4. Nagano,Y., et al. J. Biol. Chem. 278 (51), 51504-51514 (2003) ; 5. Marx,F.P., etal. Hum. Mol. Genet. 12 (11), 1223-1231 (2003) ; 6. Junn,E., et al. J. Biol. Chem. 277 (49), 47870-47877 (2002) ; 7. Chung,K.K., et al. Nat. Med. 7 (10), 1144-1150 (2001) ; 8. Kawamata,H., et al. J. Neurochem. 77 (3), 929-934 (2001) ; 9. Engelender,S., et al. Nat. Genet. 22 (1), 110-114 (1999)
USBio References
No references available
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