Mouse Anti-Syntaxin (Synaptotagmin-Associated 35kD Protein, Neuron-Specific Antigen HPC-1)
Syntaxin is membrane integrated Q-SNARE protein participating in exocytosis. Syntaxin is composed of an N-terminal regulatory domain (Habc), a SNARE domain (known as H3), and a single C-terminal transmembrane domain. The SNARE(H3) domain binds to both synaptobrevin and SNAP-25 forming the core SNARE complex.
Applications
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilutions
ELISA: 1:500 Western Blot: 1:100-1:10,000 Immunohistochemistry: 1:100-1:200. Formalin fixed (fixed <4 weeks) Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Crude synaptic immunoprecipitate (Human).
Form
Supplied as a liquid in 0.09% sodium azide.
Specificity
Recognizes human Syntaxin (34-36kD). Reacts with syntaxin expressed in bacterial systems. Species Crossreactivity: porcine, hamster and rat.
