Technical Data

T0297-PE
Clone Type
Polyclonal
Host
Rabbit
Source
Mouse
Conjugate
PE
Isotype
IgG
Grade
Affinity Purified
Applications
WB
Crossreactivity
Hu Mo
Accession #
NP_996738
Shipping Temp
Blue Ice
Storage Temp
4°C Do Not Freeze
Notes
Preservative Free
BSA Free
Rabbit Anti-TACC2 (Transforming, Acidic Coiled-coil containing protein 2, Anti-Zuai-1, AZU-1, ECTACC) (PE)

TACC2, also known as transforming acidic coiled-coil-containing protein 2, belongs to the transforming acidic coiled-coil-containing (TACC) protein family. This group of proteins is defined by its highly conserved coiled-coil domain. This domain is necessary for the assignment of the centrosome-spindle apparatus during cell cycling. There are three proteins belonging to the TACC family; TACC1, TACC2, and TACC3. TACC2 has been reported as playing a necessary role in cultured cell microtubule stabilization. It has also been shown that the binding of TACC2 by T antigen can cause chromosomal instability and mitotic defects (Tei, 2009). Research into the over expression of TACC2 strongly suggests a potential function as a tumor suppressor (Tei, 2009).

Applications
Suitable for use in Western Blot. Other applications have not been tested.
Recommended Dilutions
Western Blot: detects TACC2 on 10ug of mouse testis tissue lysate Optimal dilutions to be determined by the researcher.
Recommended Secondary Antibodies
I1904-39: IgG, X-Adsorbed (HRP) Pab Gt xRb I1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt xRb I1904-46Q: IgG, H&L (HRP) Pab Gt xRb
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
Linear peptide corresponding to the mature chain of mouse TACC2, conjugated to KLH. Species Sequence Homology: rat
Form
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
Purity
Purified by affinity chromatography.
Specificity
Recognizes mouse TACC2, Mr ~65kD. TACC2 has a reported Mr ~73kD in HeLa cell lysates. The immunizing peptide inhibited antibody recognition of the Mr ~65kD band in a competition experiment. Species Crossreactivity: human

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Gergely, F., et al., PNAS USA 97: 14352-14357 (2000). 2. Huei-Mei, C., et. al., Molecular Biology 11: 1357-1367 (2000).
USBio References
No references available
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