Mouse Anti-Tau, 4-repeat isoform RD4 (Tau Protein, Microtubule-associated Protein, MAP)
Microtubule Associated Proteins (MAPS) bind to the tubulin subunits of microtubule structures and regulate their functional stability. In the cell MAPs bind to monomer and multimerized tubulin. MAP binding to multimerized tubulin further stabilizes the formation of higher order microtubulin structures. MAP binding to microtubule structures is mediated through phosphorylation through Microtubule Affinity Regulated Kinase (MARK). Phosphorylation releases MAPs bound to microtubules, destabilizing the structure, driving it toward disassembly. There are predominately two MAP types, I, II. Type II MAP includes MAP2, MAP4 and tau and are found in nervous tissue. Six tau isoforms exist in brain tissue; they are distinguished by their number of binding domains. Three isoforms have three binding domains and the other three have four binding domains. The binding domains are located in the carboxy-terminus of the protein and are positively-charged. The isoforms with four binding domains are better at stabilizing microtubules than those with three binding domains.
Applications
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilutions
Western Blot: 1:1000-1:10,000. Detects Tau (4-repeat isoform RD4) in a rat brain cytosolic fraction. Rat brain cytosol fraction was resolved by electrophoresis, transferred to nitrocellulose and probed with T1030-40A. Proteins were visualized using an IgG (HRP) goat anti-mouse and a chemiluminescence detection system. Immunohistochemistry: Detects Tau (4-repeat isoform RD4) in autoclaved paraffin brain sections (1-2). Differential Detection of Tauopathies: See reference 2. Optimal dilutions to be determined by the researcher.
Recommended Secondary Antibodies
I1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt x Mo I1904-06H: IgG, H&L (HRP) (X-Adsorbed) Pab Gt x Mo
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to aa275-291, VQIINKKLDLSNVQSKC of human Tau (4-repeat isoform RD4) (Bovine thyroglobulin coupled). Region is flanking junction coded by adjacent exons 9 & 11 with the inclusion of exon 10.
Form
Supplied as a liquid, 0.05% sodium azide. Also available without azide. See T1030-40A1.
Specificity
Recognizes human Tau (4-repeat isoform RD4), Mr 45-65kD. Higher MW band (68-72kD) represents phosphorylated Tau. Species Crossreactivity: rat. Species Sequence Homology: mouse and bovine.
