Rabbit Anti-Tau, phosphorylated (Ser396) (Tau Protein, Microtubule-associated Protein, MAP)
Tau is a neuronal microtubule-associated protein found predominantly on axons. The function of tau is to promote tubulin polymerization and stabilize microtubules. Tau, in its hyperphosphorylated form, is the major component of paired helical filaments (PHF), the building block of neurofibrillary lesions in Alzheimer’s disease (AD) brain. Hyperphosphorylated tau is also found in neurofibrillary lesions in a range of other central nervous system disorders. Hyperphosphorylation impairs the microtubule binding function of tau, resulting in the destabilization of microtubules in AD brains, ultimately leading to the degeneration of the affected neurons. Numerous serine/threonine kinases, including GSK-3beta, protein kinase A (PKA), cyclin-dependent kinase 5 and casein kinase II, phosphorylate tau. Serine 396 is phosphorylated by GSK-3beta in vitro and in vivo.
Applications
Suitable for use in Western Blot. Other applications not tested.
Recommended Dilutions
Western Blot: 1:1000 Optimal dilutions to be determined by the researcher.
Positive Control
Recombinant human tau left untreated, or treated with GSK-3beta
Storage and Stability
May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for 12 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Synthetic phosphopeptide derived from a region of human tau that contains serine 396. Species Sequence Homology: mouse, rat, Rhesus Monkey, baboon, bovine and goat; 100%.
Form
Supplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.3, 1mg/ml BSA (IgG, protease free), 0.05% sodium azide, 50% glycerol
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human tau. Species Crossreactivity: mouse and rat
