T1041-04A
Clone Type
PolyclonalHost
RabbitSource
HumanIsotype
IgGGrade
Affinity PurifiedApplications
WBCrossreactivity
HuShipping Temp
Blue IceStorage Temp
-20°CRabbit Anti-Tau, phosphorylated (T231) (Tau Protein, Microtubule-associated Protein, MAP)
Tau is a neuronal microtubule-associated protein found predominantly on axons and functions to promote tubulin polymerization and stabilize microtubules. Tau, in its hyperphosphorylated form, is the major component of paired helical filaments (PHF), the building block of neurofibrillary lesions in Alzheimer’s disease (AD) brain. Hyperphosphorylated Tau is also found in neurofibrillary lesions in a range of other central nervous system disorders. Hyperphosphorylation impairs the microtubule binding function of Tau, resulting in the destabilization of microtubules in AD brains, ultimately leading to the degeneration of the affected neurons. Numerous serine/threonine kinases including GSK-3b, protein kinase A (PKA), cyclin-dependent kinase 5 (cdk5) and casein kinase II (CK2), phosphorylate Tau. Threonine 231 is phosphorylated by GSK-3b, cdk5 and cdk1, and has been shown to be involved in the pre-tangle process in AD.
Applications
Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution
Western Blot: 1:1000 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic phosphopeptide derived from the region of human tau that contains threonine 231. |Species sequence homology: mouse, rat
Form
Supplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.3, 1mg/ml BSA (IgG and protease free), 0.05% sodium azide, 50% glycerol.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human Tau.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
References
1. Jämsä, A., et al. (2004) Biochem. Biophys. Res. Comm. 319:993-1000. 2. Augustinack, J.C., et al. (2002) Acta Neuropathol. 103:26-35. 3. Liu, F., et al. (2002) FEBS Lett. 530(1-3):209-214. 4. Savage, M.J., et al. (2002) J. Neurosci. 22(9):3376-3385. 5. Tesseur, I., et al. (2000) Am. J. Pathol. 156(3):951-964. 6. Goedert, M. (1999) Nature. 399(6738):739-740. 7. Lu, P.J., et al. (1999) Nature 399:784-788. 8. Wang, J.Z., et al. (1998) FEBS Lett. 436(1):28-34. 9. Reynolds, C.H., et al. (1997) J. Neurochem. 68(4):1736-1744. 10. Singh, T.J., et al. (1997) Mol. Cell. Biochem. 168(1-2):141-148.USBio References
No references available