Mouse Anti-TBX21 (T-box Transcription Factor TBX21, T-box Protein 21, Transcription Factor TBLYM, T-cell-specific T-box Transcription Factor T-bet, TBET, TBLYM) (Azide Free)
This gene is a member of a phylogenetically conserved family of genes that share a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. This gene is the human ortholog of mouse Tbx21/Tbet gene. Studies in mouse show that Tbx21 protein is a Th1 cell-specific transcription factor that controls the expression of the hallmark Th1 cytokine, interferon-gamma (IFNG). Expression of the human ortholog also correlates with IFNG expression in Th1 and natural killer cells, suggesting a role for this gene in initiating Th1 lineage development from naive Th precursor cells
Applications
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilutions
Western Blot: 1:500-1:1000 ELISA: 1ug/ml-3ng/ml Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Partial recombinant protein corresponding to a portion of amino acids within aa387-486 of human TBX21 with a GST tag. The MW of the GST tag alone is 26kD.
Form
Supplied as a liquid in PBS, pH 7.2. Azide free.
Purity
Purified by ammonium sulfate precipitation.
Specificity
Recognizes human TBX21.
