Technical Data

T2009-04B1
Clone Type
Monoclonal
Host
Mouse
Source
Human
Isotype
IgG2b
Clone Number
11n183
Grade
Affinity Purified
Applications
WB
Crossreactivity
Hu
Gene ID
9466
Shipping Temp
Blue Ice
Storage Temp
4°C Do Not Freeze
Mouse Anti-TCCR (T Cell Cytokine Receptor, IL-27 R alpha, IL27RA, WSX-1)

TCCR, also known as WSX-1 and IL-27 Ra, is a 96kD member of the type I group 2 cytokine receptor family.1-4 The extracellular region of human TCCR shares 63% aa identity with the extracellular domain of mouse IL-27 Ra.2, 3 TCCR is expressed in mast cells, endothelial cells, NK cells, macrophages, monocytes, B cells, dendritic cells, and native T cells.1, 2, 4, 5 As with many other class I cytokine receptor chains, IL-27 Ra heterodimerizes with gp130, the common receptor subunit of IL-6 family cytokines, to form a functional IL-27 receptor.6 IL-27 only binds to IL-27 Ra/WSX-1/TCCR and not the gp130 subunit.7 Studies using WSX-1 mice reveal that WSX-1 has the ability to suppress T cell hyperactivity during infection, and to mediate an inhibition of both type 1 and type 2 T cell immunity.4, 8, 9 In particular, WSX-1 is believed to induce IL-12 R on native T cells, predisposing them to a Th1 phenotype. This is offset by its ability to limit IL-12 (and TNF) production by macrophages, and its suppression of IFN-g production.

Applications
Suitable for use in Flow Cytometry. Other applications not tested.
Recommended Dilution
Western Blot: 1ug/ml Optimal dilutions to be determined by the researcher.
Hybridoma
NSO myeloma cells from Balb/c mice.
Absorption: 488nm
Emission: 565-605nm
Storage and Stability
May be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. Stable for at least 12 months at 4°C. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity. PE conjugates are sensitive to light.
Immunogen
Recombinant protein corresponding to human TCCR expressed in NSO cells (Q6UWB1).
Form
Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 1ml sterile PBS.
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes human TCCR. No cross­reactivity with recombinant human gp130 or recombinant mouse IL­27 Ra was observed by direct ELISA using the unconjugated form of this antibody.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Villarino, A.V. et al. (2004) J. Immunol. 173:715. 2. Chen, Q. et al. (2000) Nature 407:916. 3. Sprecher, C.A. et al. (1998) Biochem. Biophys. Res. Commun. 246:82. 4. Artis, D. et al. (2004) J. Immunol. 173:5626. 5. Holscher, C. et al. (2005) J. Immunol. 174:3534. 6. Pflanz, S. et al. (2004) J. Immunol. 172:2225. 7. Scheller, J. et al. (2005) Biochem. Biophys. Res. Commun. 326:724. 8. Villarino, A. et al. (2003) J. Immunol. 170:645. 9. Hamano. S. et al. (2003) Immunity 19:657.
USBio References
No references available
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