Mouse Anti-Tenascin (TN, Cytotactin, GMEM, GP 150-225, Glioma-associated-extracellular Matrix Antigen, Hexabrachion, JI, Myotendinous Antigen, Neuronectin, Tenascin-C, TN-C, TNC, HXB)
The tenascins are a family of extracellular matrix proteins (ECMs). Tenascin is an extracellular matrix protein. It expressed in an unusually restricted pattern during embryogenesis and has been implicated in a variety of morphogenetic phenomena. Human hexabrachion gene (tenascin) is mapped to chromosome 9, bands q32-q34. The coding region of the hexabrachion gene spans approximately 80 kilobases of DNA and consists of 27 exons separated by 26 introns.
Applications
Suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested.
Recommended Dilutions
Western Blot: 1-2ug/ml Immunohistochemistry: 2-4ug/ml using acetone fixed tissues and formalin fixed and paraffin embedded tissues. Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Form
Supplied as a lyophilized powder from 1.2% sodium acetate, 2% BSA, 0.01% sodium azide. Reconstitute with 1ml sterile PBS.
Specificity
Recognizes human Tenascin.
