T2951-ML490
Clone Type
MonoclonalHost
MouseConjugate
MaxLight™490Isotype
IgGClone Number
M021812Grade
Highly PurifiedApplications
FLISA RIAShipping Temp
Blue IceStorage Temp
4°C Do Not FreezeNotes
Preservative Free
BSA Free
Mouse Anti-Testosterone (MaxLight 490)
MaxLight™ 490 is a new Blue-Green photostable dye conjugate comparable to DyLight™488, Alexa Fluor™488 and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (491nm); Emission (515nm); Extinction Coefficient 73,000.
Testosterone is a steroid hormone from the androgen group and is found in mammals, reptiles, birds, and other vertebrates. In mammals, testosterone is primarily secreted in the testes of males and the ovaries of females, although small amounts are also secreted by the adrenal glands. It is the principal male sex hormone and an anabolic steroid.
Applications
Suitable for use in FLISA and RIA. Other applications not tested.
Recommended Dilutions
Optimal dilutions to be determined by the researcher.
Affinity Constant
2.5x10e9L/mole
Contaminants
DHT; 9% Androstendiol; <1% Androstendione, Estriol, Estradiol, Progesterone: <0.1%
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
Testosterone-3-BSA
Form
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™490.
Purity
Purified by Ion Exchange chromatography
Specificity
Recognizes Testosterone.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
References
1. Karen L. Portera, Allen W. Olmstead, David M. Kumsher, William E. Dennis, Robert L. Sprando, Gary W. Holcombe, Joseph J. Korte, Annelie Lindberg-Livingston, Sigmund J. Degitz. Effects of 4-tert-octylphenol on Xenopus tropicalis in a long term exposure. Aquatic Toxicology, Volume 103, Issues 3-4, Pages 159-169. 2. Troy A. Bairda, Diana K. Hews. Hormone levels in territorial and non-territorial male collared lizards. Physiology & Behavior, Volume 92, Issue 4, Pages 755-763. 3. Brent M. Hortona, Jongmin Yoonb, Cameron K. Ghalamborb, Ignacio T. Moorec, T. Scott Sillett. Seasonal and population variation in male testosterone levels in breeding orange-crowned warblers (Vermivora celata). General and Comparative Endocrinology, Volume 168, Issue 3, Pages 333-339. 4. Allen W. Olmstead, Joseph J. Korte, Kacie K. Woodis, Blake A. Bennett, Shannon Ostazeski, Sigmund J. Degitz. Reproductive maturation of the tropical clawed frog: Xenopus tropicalis. General and Comparative Endocrinology, Volume 160, Issue 2, Pages 117-123. 5. Ke-Zhong Lianga, Jun-Sheng Qia, Wei-Jun Mub, Zai-Gang Chen. Biomolecules/gold nanowires-doped sol-gel film for label-free electrochemical immunoassay of testosterone. Journal of Biochemical and Biophysical Methods, Volume 70, Issue 6, Pages 1156-1162USBio References
No references available