Goat Anti-TFCP2L1 (Transcription Factor CP2-like 1, CP2-related Transcriptional Repressor 1, CP2-related Transcriptional Repressor-1, CRTR1, CRTR-1, LBP9, LBP-9, Transcription Factor CP2-like Protein 1, Transcription Factor LBP-9)
TFCP2L1 (Transcription factor CP2-like protein # 1; also LBP-9) is a 54kD (predicted) member of the CP2 subfamily, grh/CP2 family of proteins. It is expressed in placental synctiotrophoblast cells, and regulates the production of CYP11A1/P450scc. P450scc is a cholesterol cleavage enzyme that generates pregnenolone, a necessary intermediate in the formation of progesterone. TFCP2L1 both positively and negatively regulates P450scc synthesis through homodimerization and heterodimerization with LBP-1b. Human TFCP2L1 is 479 amino acids (aa) in length. It contains a transcriptional repressor region (aa 100-200) and a dimerization domain (aa 300-479). There is one splice variant that shows a deletion of aa 400-464. Human TFCP2L1 is 73% aa identical to human TFCP2, and 93% aa identical to mouse CRTR-1, a closely related molecule that may not represent the actual mouse ortholog to TFCP2L1.
Applications
Suitable for ELISA and Western Blot.Other Applcations not tested.
Western Blot
Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
E. coli-derived recombinant protein corresponding to Met1-Lys180 of human TFCP2L1.
Form
Supplied as a lyophilized powder in PBS, pH 7.2, with 5% trehalose. Reconstitute with 500ul sterile PBS
Purity
Purified by human TFCP2L1 affinity chromatography.
Specificity
This antibody has been selected for its ability to recognize human TFCP2L1 in direct ELISAs and Western blots.
