Rabbit Anti-THEM2 (Thioesterase Superfamily Member 2, Acyl-coenzyme A Thioesterase 13, Acyl-CoA Thioesterase 13, ACOT13, HT012, MGC4961, PNAS-27)
EC=3.1.2.-
THEM2 is a 140aa enzyme with hydrolase activity belonging to the thioesterase superfamily and UPF0152 (paal) family. Human THEM2 contains a hotdog-fold and forms a back-to-back tetramer with the active site located in an inter-subunit cleft. The biological function of THEM2 is yet unknown. The role of THEM2 and Pax3 (paired box gene 3) as PC-TP-interacting proteins has been determined by using yeast two-hybrid screening. Small interference RNA silencingDta suggest that the gene encoding THEM2 is essential for the cell sustained proliferation. THEM2 is highly expressed in kidney, and moderately in liver, brain, large intestine, and small intestine. THEM2 is colocalized with microtubules and is implicated in the genetic background of developmental dyslexia.
Applications
Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution
Western Blot: 0.05-0.1ug/ml Optimal dilutions to be determined by the researcher.
Positive Control
293 or RAW cell lysate
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to aa100-150 of human THEM2.
Form
Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes human THEM2. Species Crossreactivity: chimpanzee, orangutan, rat. Species sequence homology: chicken, bovine, canine, mouse, opossum, xenopus, zebrafish.
