Technical Data

T5005-21
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
E
Crossreactivity
Hu
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-Thiamine Transporter Protein 1

A sodium-dependent transport system is responsible for transfer and distribution of vitamins to different parts of the body, the transfer includes vitamins like pantothenate, biotin, and ascorbic acid etc, These transporters belong to Solute Carrier family (SLC). Since vitamins are required for essential metabolic processes in all mammalian cells, such cells have developed intrinsic mechanisms to active accumulation of essential vitamins. Thus transporters help these cells to fulfill their requirement, they include Sodium-dependent Multi-Vitamin Transporter (SMVT), Sodium-dependent Vitamin-C Transporter (SVCT) 1 & 2, Creatine Transporter (CRT1/ CT1). The other vitamin transporters from SLC family include Thiamine Transporter Protein 1 (THTR1), Folate Transporter or Reduced Folate Carrier 1 (RFC1), Thyroid Iodide Transporter (TIT) and Taurine Transporter (TAU) etc. Thiamine Transporter Protein 1 (THTR1),a 498aa protein in mouse and 497 in human (chrm 1q23.3, gene SLC19A2) is a high affinity transporter for the intake of Thiamine, Most abundantly found in skeletal and cardiac muscle, lower levels are seen in placenta, heart, liver and kidney. Defects in gene are the cause of Thiamine Responsive Megaloblastic Anemia (TRMA).

Applications
Suitable for use in ELISA. Western Blot, though not tested, may potentially be used as an application. Other applications not tested.
Recommended Dilution
ELISA: 0.5-1ug/ml; Control peptide can be used to coat ELISA plates at 1ug/ml. See T5005-21A. Western Blot: 1-10ug/ml using ECL technique. Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
16aa peptide from human THTR1. Location: ~C-terminus, Cytoplasmic domain. Species Sequence Homology: Mouse - 68%.
Form
Supplied as a lyophilized powder from PBS, 0.05% sodium azide.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human Thiamine Transporter Protein 1.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
Fleming J. C et al (1999) Nature genetics 22 (3) 305-308, Fleming J. C et al (1999) direct submission, Hematology, Children’s Hospital/ Harvard Med School, Boston, MA.
USBio References
No references available
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