Technical Data

T5300-17F
Clone Type
Monoclonal
Host
Mouse
Source
Human
Isotype
IgG1
Clone Number
2H11
Grade
Affinity Purified
Applications
IHC
Crossreactivity
Hu
Shipping Temp
Blue Ice
Storage Temp
-20°C
Mouse Anti-Thyroglobulin (Tg, Tgn, AITD 3, AITD3, hTG)

Thyroglobulin is the glycoprotein precursor of the iodinated thyroid hormones thyroxine (T4) and triiodothyronine (T3). Thyroglobulin is obtained from the thyroid gland and exhibits the general properties of the globulins. The human thyroglobulin (hTG) is a high molecular weight glycoprotein (605kD) found in the thyroid follicular cells. It plays a central role in the uptake, incorporation, and regulated biosynthesis of thyroid hormones, T4 and T3. Thyroid disorders are, in large part, due to autoimmune origin, and anti thyroglobulin autoantibodies were the first factor to be discovered. Anti hTG is found in all thyroid autoimmune diseases (Hashimoto’s thyroiditis, Graves’s diseases), with the highest level observed in Hashimoto’s thyroiditis. Anti hTG is also characteristic of thyroid cancer, and its determination can be used for the follow up of cancer patients. TG is mainly expressed on thyroid follicular cells (99.1%).

Applications
Suitable for use in Immunohistochemistry. Other applications not tested.
Recommended Dilutions
Immunohistochemistry (paraffin sections): 1:80 -1:100; pretreatment: heat retrieval in 0.01M citrate buffer, pH 6.o (microwave oven). Positive tissue: thyroid gland, follicular adenoma. Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Thyroid follicular cells
Form
Supplied as liquid in PBS, pH 7.4, 15mM sodium azide.
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes human thyroglobulin.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Emerson CH & Torres MS Recombinant human thyroid-stimulating hormone: pharmacology, clinical applications and potential uses. BioDrugs 17:19-38 (2003). PubMed: 12534318|2. Lazar V et al. Expression of the Na+/I- symporter gene in human thyroid tumors: a comparison study with other thyroid-specific genes. J Clin Endocrinol Metab 84:3228-34 (1999). PubMed: 10487692|3. Hishinuma A et al. Two novel cysteine substitutions (C1263R and C1995S) of thyroglobulin cause a defect in intracellular transport of thyroglobulin in patients with congenital goiter and the variant type of adenomatous goiter. J Clin Endocrinol Metab 84:1438-44 (1999). PubMed: 10199792
USBio References
No references available
United States Biological | 4 Technology Way | Salem, MA 01970
Phone 800-520-3011 | Fax 978-594-8052 | Website www.usbio.net