Technical Data

T5469-91F
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
WB
Crossreactivity
Hu Mo
Accession #
EAW61615.1; NP_036338
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-TIM-1 (T Cell Immunoglobin Domain and Mucin Domain Protein 1, TIM 1, TIM1, TIMD 1, TIMD1, TIMD-1, Hepatitis A Virus Cellular Receptor 1, HAVCR 1, HAVCR1, HAVCR-1, Kidney Injury Molecule 1, KIM1, KIM-1)

TIM-1, also known as HAVcr1 (C Hepatitis A virus cellular receptor 1) and KIM-1 (Kidney Injury Molecule 1), is a 60-100kD member of the TIM family of immune regulating adhesion proteins. The mature molecule is a type I transmembrane glycoprotein that contains a 214aa extracellular domain (ECD) and a 51aa cytoplasmic tail. The ECD contains one Ig-like V-type domain, a Ser/Thr-rich mucin region, and multiple glycosylation sites. TIM-1 is involved in the development of Th2-biased immune responses, and the presence of particular TIM-1 mutations correlates with the susceptibility to allergic disease. TIM-1 binds TIM-4 and serves as the receptor for the hepatitis A virus. It is also upregulated in renal epithelial cells following tissue injury. The extracellular domain of TIM-1 can be shed and serves as an indicator of kidney tubule damage. Within the ECD, rat TIM-1 shares 81% and 51% aa sequence identity with mouse and human TIM-1, respectively.

Applications
Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution
Western Blot: 1-3ug/ml Optimal dilutions to be determined by the researcher.
Positive Control
Human or mouse brain tissue lysate
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide (SPLYSYTTDGNDTVT) corresponding to aa248-262 of human NP_036338 and aa84-98 of human EAW61615.1.
Form
Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.
Purity
Purified by Protein G affinity Chromatography.
Specificity
Recognizes human HAVCR1/TIM-1. Species Crossreactivity: mouse. Species sequence homology: monkey.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Feigelstock,D., Thompson,P., Mattoo,P., Zhang,Y. and Kaplan,G.G. The human homolog of HAVcr-1 codes for a hepatitis A virus cellular receptor. J. Virol. 72 (8), 6621-6628 (1998). 2. Vila,M.R., Kaplan,G.G., Feigelstock,D., Nadal,M., Morote,J., Porta,R., Bellmunt,J. and Meseguer,A. Hepatitis A virus receptor blocks cell differentiation and is overexpressed in clear cell renal cell carcinoma. Kidney Int. 65 (5), 1761-1773 (2004). 3. McIntire,J.J., Umetsu,D.T. and DeKruyff,R.H. TIM-1, a novel allergy and asthma susceptibility gene. Springer Semin. Immunopathol. 25 (3-4), 335-348 (2004). 4. Chae,S.C., Song,J.H., Heo,J.C., Lee,Y.C., Kim,J.W. and Chung,H.T. Molecular variations in the promoter and coding regions of human Tim-1 gene and their association in Koreans with asthma. Hum. Immunol. 64 (12), 1177-1182 (2003). 5. McIntire, JJ et al. Nature Immunol. 2, 1109-1116 (2001) 6. McIntire, JJ et.al. Nature 425(6958):576 (2003 Oct 9) 7. V.Bailly et.al J. Biol. Chem., Vol. 277, Issue 42, 39739-39748 (October 18, 2002) 8. Umetsu D, S Umetsu, GJ Freeman, RH Dekruyff. TIM gene family and their role in atopic disease. Curr Top Microbiol Immunol 208:201-215. (2008).
USBio References
No references available
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