T5469-92Q
Clone Type
MonoclonalHost
MouseSource
HumanConjugate
PEIsotype
IgG1,kClone Number
11E365 (F38-2E2)Grade
Affinity PurifiedApplications
FCCrossreactivity
HuAccession #
NP_116171Gene ID
84868Shipping Temp
Blue IceStorage Temp
4°C Do Not FreezeMouse Anti-Tim3 (T Cell Immunoglobulin and Mucin Domain Containing Protein 3, Hepatitis Virus Cellular Receptor 2, CD366) (PE)
CD366 (Tim-3) is a transmembrane protein also known as T cell immunoglobulin and mucin domain containing protein-3. Tim-3 is expressed at high levels on activated T cells (preferentially on Th1 cells, monocytes/macrophages, and dendritic cells). Tim-3 has also been shown to exist as a soluble protein. Cells expressing Tim-3 are present at high levels in the CNS of animals at the onset of experimental autoimmune encephalomyelitis (EAE), a disease mediated by lymphocytes secreting Th1-like cytokines. Tim-3 has been proposed to inhibit Th1-mediated immune responses and promote immunological tolerance.
Applications
Suitable for use in Flow Cytometry. Other applications not tested.
Recommended Dilutions
Flow Cytometry: 5ul/10e6 cells in 100ul Optimal dilutions to be determined by the researcher.
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Immunogen
Human Tim-3 fusion protein
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide and BSA. Labeled with R-Phycoerythrin (PE).
Purity
Purified by affinity chromatography.
Specificity
Recognizes human TIM-3.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
References
1. Hastings, W.D. et.al. 2009 Eur. J. Immunol. 39: 2492. 2. Hafler,D.A and V. Kuchroo, et.al. 2008 J. Exp. Med. 205:2699. 3. Zhu, C et.al 2005 Nat. Immunol 6:1245. 4. Su, E.W. Lin, V, and L.P. Kane 2008 Cytokine 44: 9. 5. Kuchroo, V.K. et.al. 2003 Nat. Rev. Immunol.3:454. 6. Monney, L. et al. 2002. Nature 415: 536.USBio References
No references available