Rabbit Anti-TICAM1, CT (TIR Domain-containing Adapter Molecule 1, TICAM-1, Proline-rich, Vinculin and TIR Domain-containing Protein B, Putative NF-kappa-B-activating Protein 502H, Toll-interleukin-1 Receptor Domain-containing Adapter Protein Inducing Interferon beta, TIR Domain-containing Adapter Protein Inducing IFN-beta, PRVTIRB, TRIF)
TRIF is a member of the Toll/interleukin-1 receptor (TIR) family, a group of proteins that include the Toll-like receptors (TLRs). TLRs are signaling molecules that recognize different pathogen-associated molecular patterns (PAMPs) and serve as an important link between the innate and adaptive immune responses. TRIF, along with other molecules such as TIRP, TIRAP, and MyD88, serves as an adaptor protein to several of the TLR molecules. Following activation of TLR3 and TLR4, TRIF engages the kinase TBK1 and allows its subsequent activation of the interferon regulatory factor (IRF)-3. TRIF is also involved in the activation of TNF receptor associated factor (TRAF)-6, and ultimately the activation of NF-kB.
Applications
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilution
Western Blot: 2-4ug/ml Immunohistochemistry (Formalin fixed paraffin embedded): 5ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to 14aa from human TRIF at C-terminal (KLH).
Form
Supplied as a liquid in PBS, 0.02% sodium azide.
Purity
Purified by Ion Exchange chromatography.
Specificity
Recognizes human TRIF.
