T5499-88C
Clone Type
PolyclonalHost
GoatSource
HumanIsotype
IgGGrade
Affinity PurifiedApplications
E WBCrossreactivity
Bo Ca Hu Mo RtAccession #
NP_690866.1Gene ID
261726, 226591, 360869Shipping Temp
Blue IceStorage Temp
-20°CGoat Anti-TIPRL (TIP41-like Protein, Putative MAPK-activating Protein PM10, Type 2A-interacting Protein, TIP)
May be a allosteric regulator of serine/threonine-protein phosphatase 2A (PP2A). Isoform 1 inhibits catalytic activity of the PP2A(D) core complex in vitro. The PP2A(C):TIPRL complex does not show phosphatase activity. May play a role in the regulation of ATM/ATR signaling pathway controlling DNA replication and repair.
Applications
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution
ELISA: 1:16,000 Western Blot: 1-3ug/ml, lysates of cell line K562 observed on ~30kD bands Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to C-RIDPNPADSQKSTQ, from human TIPRL, at C-terminal (NP_690866.1).
Form
Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human TIPRL. Species sequence homology: Bovine, canine, mouse and rat.
