Technical Data

T5585-10W
Clone Type
Polyclonal
Host
Goat
Source
Zebrafish
Isotype
IgG
Grade
Affinity Purified
Applications
E
Crossreactivity
Ze
Accession #
NP_878294.1
Shipping Temp
Blue Ice
Storage Temp
-20°C
Goat Anti-Timp2 (Tissue Inhibitor of Metalloproteinase 2, timp2a)

Tissue inhibitors of metalloproteinases (TIMPs) are involved in the control of matrix-metalloproteinase (MMP) activity as a key inhibitor in tissue. Expression of MMPs and TIMPs are controlled during tissue remodeling to maintain a balance in the regulation of extracellular matrix turnover and other morphogenic processes. TIMP-2 has also been reported to have growth factor properties. Zebrafish TIMP-2 shows high similarity to human TIMP-2 with all critical features conserved. Immunoreactive zebrafish TIMP-2 was detected at the protein level with a recombinant antibody for human TIMP-2. TIMP-2 was shown to be expressed as early as the one cell stage, and blockage of TIMP-2 translation resulted in abnormal zebrafish development.

Applications
Suitable for use in ELISA. Other applications not tested.
Recommended Dilution
ELISA: 1:16,000 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to C-TEKIIHGRQSDH, from timp2, at the internal region of the protein (NP_878294.1).
Form
Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes Timp2. Species sequence homology: Zebrafish.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Zhang J, Bai S, Tanase C, Nagase H, Sarras MP Jr. The expression of tissue inhibitor of metalloproteinase 2 (TIMP-2) is required for normal development of zebrafish embryos. Dev Genes Evol. 2003 Aug;213(8):382-9. Epub 2003 May 8.
USBio References
No references available
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