T5650
Clone Type
MonoclonalHost
MouseSource
HumanIsotype
IgG1Clone Number
2Q1063Grade
SupernatantApplications
IHC WBCrossreactivity
Hu Mo RbAccession #
NM_003319.3,|NM_133378.3,|NM_133379.2,|NM_133432.2,|NM_133437.2Shipping Temp
Blue IceStorage Temp
-20°CNotes
BSA Free
Mouse Anti-Titin (Connectin, TTN)
Connectin/Titin is an ~ megadalton (MD) elastic filamentous protein of striated muscle sarcomeres. One connectin molecule spans from the Z line to the M line on the myosin filament in a half sarcomere, thus forming the third filament in addition to the myosin and actin filaments in a sarcomere. Connectin filaments position the myosin filaments at the center of a sarcomere as a spring and are responsible for passive tension generation when sarcomeres are stretched.
Applications
Suitable for use in Immunohistochemistry and Western Blot. Other applications not tested.
Recommended Dilutions
Immunohistochemistry: Neat to 1:10 Western Blot: 1:10-1:20 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Electrophoretically purified human skeletal muscle Titin.
Form
Supplied as a liquid, 0.09% sodium azide.
Specificity
Recognizes human skeletal muscle Titin. Species Crossreactivity: rabbit and mouse
