Technical Data

T7948-20
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
WB
Crossreactivity
Bo Ca Ch Eq Hu Mo
Accession #
NP_057356.1
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-TOB2 (Protein Tob2, Transducer of erbB-2 2, Protein Tob4, KIAA1663, TOB4, TOBL, TROB2)
Transducer of ERBB2, 2

TOB2 is a 344aa rich novel member of the Tob:BTG1 anti-proliferative family of proteins characterized by similarities in their N-terminal BTG:Tob homology domains. This anti-proliferative protein inhibits cell cycle progression from the G0:G1 to S phases and is involved in cell cycle regulation through interaction with Caf1, a component of the CCR4 transcription factor complex that associates with cyclin-dependent kinases. Recent reports suggest that TOB2 plays important roles in spermatogenesis, embryonic dorsoventral patterning, osteogenesis, T-cell activation, learning and memory and acts primarily as a transcriptional repressor in several signaling pathways. TOB2, a signaling protein, may participate in transcriptional regulation of several genes. It is a regulatory protein ubiquitously expressed in most of the tissues.

Applications
Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution
Western Blot: 0.5-1ug/ml Optimal dilutions to be determined by the researcher.
Positive Control
Human heart lysate
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to aa50-100 of human TOB2.
Form
Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes human TOB2. Species Crossreactivity: chicken, chimpanzee, bovine, canine, equine, mouse, orangutan. Species sequence homology: xenopus, zebrafish.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Ajima, R. et al. Gene. 253:215-220 (2000). 2. Ikematsu, N. et al. Oncogene. 18:7432-7441, 1999. 3. Jia, S. et al. Dev Dyn. 236:913-921 (2007).4. Kawamura-Tsuzuku, J. et al. Oncogene. 23:6630-6638 (2004).
USBio References
No references available
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