T8050-90A
Clone Type
MonoclonalHost
MouseSource
HumanIsotype
IgG1,kClone Number
11E377 (26C593.2)Grade
Affinity PurifiedApplications
FC IC IF IHC WBCrossreactivity
Ca Eq Hu Mk Mo RtAccession #
AAF72190, NP_059138Shipping Temp
Blue IceStorage Temp
-20°CNotes
Preservative Free
Mouse Anti-TLR9 (Toll-like Receptor 9, CD289, UNQ5798/PRO19605) (Azide Free)
The Toll-like receptor (TLR) family in mammal comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules. The TLR family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition. Ten human homologs of TLRs (TLR1-10) have been described. By using a BLAST search, Hemmi et al., 2000 have identified and subsequently isolated a cDNA coding for TLR9. Gene knockout experiments suggest that TLR9 acts as a receptor for unmethylated CpG dinucleotides in the bacterial DNA. Human and mouse TLR9 share an overall aa identity of 75.5%. TLR9 is highly expressed in spleen.
Applications
Suitable for use in Immunofluorescence, Flow Cytometry, Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested.
Recommended Dilutions
Flow Cytometry: 0.5-2ug/10e6 cells Western Blot: 2-5ug/ml Immunofluorescence (IC): 1:10-1:500 Immunohistochemistry (Paraffin): 5ug/ml. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10mM sodium citrate buffer, pH 6.0 for 10-20 minutes followed by cooling at RT for 20 minutes Optimal dilutions to be determined by the researcher.
Positive Controls
Western Blot: Human PBMC or human intestine cell lysate Flow Cytometry: Ramos cells
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
KLH-conjugated synthetic peptide corresponding to aa268-300 of human TLR9 isoform A.
Form
Supplied as a liquid in PBS. No preservative added.
Purity
Purified by Protein G affinity chromatography.
Specificity
Recognizes human TLR9. Species Crossreactivity: canine, mouse, rat, Rhesus monkey, equine and primates
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
References
1. Muzio M, Natoli G, Saccani S, Levrero M, and Mantovani A. J. exp. Med. 187: 2097-2101 (1998). 2. Medzhitov R and Janeway CA. Cell 91: 295-298 (1997). 3. Chuang TH and Ulevitch RJ. Biochim. Biophys. Acta 1518 (1-2):157-161 (2001) 4. Takeuchi O, Kawai T, Sanjo H, Copeland NG, Gilbert DJ, Jenkins NA, Takeda K, and Akira S. Gene 231: 59-65 (1999). 5. Hemmi H, Takeuchi O, Kawai T, Kaisho T, Sato S, Sanjo H, Matsumoto M, Hoshino K, Wagner H, Takeda K, and Shizo A. Nature 408: 740-745 (2000).USBio References
No references available