Technical Data

T8067-55A
Clone Type
Monoclonal
Host
Rabbit
Source
Human
Isotype
IgG
Clone Number
8A39(C71D11)
Grade
Purified
Applications
WB
Crossreactivity
Hu Mo Rt
Gene ID
23373
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-Torc1 (Transducer of Regulated CREB Activity 1)

Glucose homeostasis is regulated by hormones and cellular energy status. Elevations of blood glucose during feeding stimulate insulin release from pancreatic b-cells through a glucose sensing pathway. Feeding also stimulates release of gut hormones such as glucagon-like peptide-1 (GLP-1), which further induces insulin release, inhibits glucagon release and promotes b-cell viability. CREB-dependent transcription likely plays a role in both glucose sensing and GLP-1 signaling. The protein Torc2 (transducer of regulated CREB activity 2) functions as a CREB co-activator and is implicated in mediating the effects of these two pathways. In quiescent cells, Torc2 is phosphorylated at Ser171 and becomes sequestered in the cytoplasm via an interaction with 14-3-3 proteins. Glucose and gut hormones lead to the dephosphorylation of Torc2 and its dissociation from 14-3-3 proteins. Dephosphorylated Torc2 enters the nucleus to promote CREB-dependent transcription. Torc2 plays a key role in the regulation of hepatic gluconeogenic gene transcription in response to hormonal and energy signals during fasting. Torc1 and Torc3, a pair of Torc2-related proteins, also act as CREB coactivators. Torc1, Torc2 and Torc3 associate with the HTLV Tax protein to promote Tax-dependent transcription of HTLV-1 long terminal repeats. CRTC1/TORC1 is highly phosphorylated at Ser151 in mouse hypothalamic cells under basal conditions. When these cells are exposed to cAMP or a calcium activa- tor, CRTC1/TORC1 is dephosphorylated and translocates into the nucleus. CRTC1/TORC1 is essential for energy balance and fertility.

Applications
Suitable for use in Western Blot. Other applications not tested.
Recommended Dilutions
Western Blot: 1:1000 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to residues surounding Val463 of human Torc1 protein Species Sequence Homology: human
Form
Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol
Purity
Purified
Specificity
Recognizes endogenous levels of total Torc1 protein. Species Crossreactivity: rat and mouse

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Hinke, S.A. et al. (2004) J. Physiol. 558, 369-380. 2. Conkright, M.D. et al. (2003) Mol. Cell 12, 413-423. 3. Iourgenko, V. et al. (2003) Proc. Natl. Acad. Sci. USA 100, 12147-12152. 4. Screaton, R.A. et al. (2004) Cell 119, 61-74. 5. Koo, S.H. et al. (2005) Nature 437, 1109-1111. 6. Koga, H. et al. (2004) J. Biol. Chem. 279, 52978-52983. 7. Siu, Y.T. et al. (2006) J. Virol. 80, 7052-7059. 8. Altarejos, J.Y. et al. (2008), Nat Med 14, 1112-1117.
USBio References
No references available
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