Technical Data

T8100-05H
Clone Type
Monoclonal
Host
Mouse
Source
Human
Isotype
IgG1,k
Clone Number
18D5
Grade
Affinity Purified
Applications
IC WB
Crossreactivity
Hu
Shipping Temp
Blue Ice
Storage Temp
-20°C
Mouse Anti-TPX2 (Targeting Protein for Xklp2, Differentially Expressed in Cancerous and Non-cancerous Lung Cells 2, DIL-2, Hepatocellular Carcinoma-associated Antigen 519, Protein fls353, Restricted Expression Proliferation-associated Protein 100, p100, C20orf1, C20orf2, DIL2, HCA519)

The TPX2 protein, also known as targeting protein for Xklp1, restricted expression proliferation associated protein p100, and differentially expressed in cancer and non-cancerous lung cells protein 2 (DIL2), is a 100kD nuclear protein that contains two coiled-coil domains. The TPX2 protein is strictly associated with spindle pole and mitotic spindle during mitosis. In the G2/S position of the cell cycle, TPX2 is diffusely distributed throughout nucleus. TPX2 has been shown to be highly expressed in lung carcinomas cell lines, but not in normal lung tissues. TPX2 is thought to be required for the Ran-GTP dependent assembly of microtubules around chromosomes required to generate stable bipolar spindle with overlapping anti-parallel microtubule arrays and may also be involved in targeting Aurora-A kinase to the mitotic spindle. TPX2 has been shown to interact with a large number of proteins including serine/threonine protein kinase 6, ribosomal protein 6, Bop-1, a-tubulin, and nucleolin among others. TPX2 can be modified by phosphorylation on serine 738.

Applications
Suitable for use in Western Blot and Immunocytochemistry . Other applications not tested.
Recommended Dilution
Western Blot: 0.2-1ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Amino acids 1-220 from human TPX2.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by affinity chromatography.
Specificity
Recognizes human TPX2.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Gruss OJ, et al. 2002. Nature Cell Biol. 4:871. 2. Heidebrecht HJ, et al. 1997. Blood 90:226. 3. Kufer TA, et al. 2002. J. Cell Biol. 158:617.
USBio References
No references available
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