Sheep Anti-Transcription Factor 7-like 1 (TCF7L1, HMG Box Transcription Factor 3, T Cell Factor 3, TCF3, TCF-3)
Transcription factor 7-like 1 (TCF7L1; also HMG box transcription factor 3, TCF-3) is a 63kD member of the TCF/LEF family of proteins. Human TCF7L1 is 588 aa in length and contains one HMG box DNA-binding domain (aa346-414). Human TCF7L1 shares 95% aa sequence identity with mouse TCF7L1. TCF7L1 is expressed in hair follicles and skin keratinocytes, and at lower levels in stomach epithelium. Functionally, it participates in the Wnt signaling pathway. TCF7L1 binds to DNA and acts as a repressor in the absence of CTNNB1, and as an activator in its presence. TCF7L1 is also necessary for the terminal differentiation of epidermal cells, the formation of keratohyalin granules and the development of the barrier function of the epidermis.
Applications
Suitable for use in Western Blot. Other applications not tested.
Recommended Dilutions
Western Blot: 0.2ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS, 0.02% NaN3. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
E.coli-derived recombinant human TCF7L1 (rhTCF7L1; aa429-581; Accession # Q9HCS4).
Form
Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human TCF7L1.
