Technical Data

T8195-61B
Clone Type
Monoclonal
Host
Mouse
Source
Human
Isotype
IgG1
Clone Number
8C336
Grade
Affinity Purified
Applications
WB
Crossreactivity
Hu
Accession #
NM_003223
Shipping Temp
Blue Ice
Storage Temp
-20°C
Mouse Anti-Transcription Factor AP-4 (TFAP4, Activating Enhancer Binding Protein 4)

Transcription factor AP4 is a member of the basic-helix-loop-helix (bHLH) transcription factors, which bind to the E-box sequence in the promoters of their target genes. AP-4 has been shown to act both as a repressor and an activator for different target genes.

Applications
Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution
Western Blot: 1:1000-1:2000 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Partial recombinant protein corresponding to human Transcription Factor AP-4
Form
Supplied as a liquid in PBS, pH 7.4, 1% BSA, 0.05% sodium azide.
Purity
Purified by Protein G affinity chromatography from hybridoma culture supernatant
Specificity
Recognizes human Transcription Factor AP-4.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
General References:|1. Hu YF, Lüscher B, Admon A, et al. (1991). "Transcription factor AP-4 contains multiple dimerization domains that regulate dimer specificity.". Genes Dev. 4 (10): 1741–52. doi:10.1101/gad.4.10.1741. PMID 2123466.|2. Mermod N, Williams TJ, Tjian R (1988). "Enhancer binding factors AP-4 and AP-1 act in concert to activate SV40 late transcription in vitro.". Nature 332 (6164): 557–61. doi:10.1038/332557a0. PMID 2833704.|3. Ou SH, Garcia-Martínez LF, Paulssen EJ, Gaynor RB (1994). "Role of flanking E box motifs in human immunodeficiency virus type 1 TATA element function.". J. Virol. 68 (11): 7188–99. PMID 7933101.|4. Lewis TB, Wood S, Michaelis EK, et al. (1996). "Localization of a gene for a glutamate binding subunit of a NMDA receptor (GRINA) to 8q24.". Genomics 32 (1): 131–3. doi:10.1006/geno.1996.0088. PMID 8786101.|5. Petersenn S, Rasch AC, Heyens M, Schulte HM (1998). "Structure and regulation of the human growth hormone-releasing hormone receptor gene.". Mol. Endocrinol. 12 (2): 233–47. doi:10.1210/me.12.2.233. PMID 9482665.|6. King-Jones K, Korge G, Lehmann M (1999). "The helix-loop-helix proteins dAP-4 and daughterless bind both in vitro and in vivo to SEBP3 sites required for transcriptional activation of the Drosophila gene Sgs-4.". J. Mol. Biol. 291 (1): 71–82. doi:10.1006/jmbi.1999.2963. PMID 10438607.|7. Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences.". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMID 12477932.|8. Beausoleil SA, Jedrychowski M, Schwartz D, et al. (2004). "Large-scale characterization of HeLa cell nuclear phosphoproteins.". Proc. Natl. Acad. Sci. U.S.A. 101 (33): 12130–5. doi:10.1073/pnas.0404720101. PMID 15302935.|9. Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMID 15489334.|10. Martin J, Han C, Gordon LA, et al. (2005). "The sequence and analysis of duplication-rich human chromosome 16.". Nature 432 (7020): 988–94. doi:10.1038/nature03187. PMID 15616553.|11. Olsen JV, Blagoev B, Gnad F, et al. (2006). "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks.". Cell 127 (3): 635–48. doi:10.1016/j.cell.2006.09.026. PMID 17081983.
USBio References
No references available
United States Biological | 4 Technology Way | Salem, MA 01970
Phone 800-520-3011 | Fax 978-594-8052 | Website www.usbio.net