Rabbit Anti-Transferrin (Biotin)
Transferrin is a single polypeptide chain glycoprotein. It is a member of the iron binding family of proteins. It has a molecular weight of 79.5kD and a serum concentration range of 1800-2700mg/L. It is synthesized in the liver. It consists of two domains each having a high affinity reversible binding site for Fe3+. The iron is transported in blood and interstitial fluids to sites of use and disposal. Iron/transferrin is essential in hemoglobin synthesis and for certain types of cell division. Serum concentration rises in iron deficiency and pregnancy. Serum concentration falls in iron overload, infection and inflammatory conditions.
Biotin/Protein Ratio
10-20 BAC molecules per rabbit IgG molecule
Applications
Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunomicroscopy. Other applications not tested.
Recommended Dilution
ELISA: 1:15,000-1:70,0000
Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Transferrin [Human Serum]
Form
Supplied as a liquid in PBS, pH 7.2, 10mg/ml polyethylene glycol (PEG-8000), 0.01% sodium azide. Labeled with Biotin.
Purity
Purified from monospecific antiserum by delipidation, salt fractionation and ion exchange chromatography.
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-rabbit serum, purified and partially purifed Transferrin.