Rat Anti-Transferrin Receptor (CD71, TR, Tfr, TfnrR)
Applications
Suitable for use in Flow Cytometry, Immunohistochemistry and Immunoprecipitation. Other applications have not been tested.
Recommended Dilution
Flow Cytometry: 1:50-1:100. 10ul to label 1x10e6 cells in 100ul. Immunohistochemistry: Frozen sections
Optimal dilutions to be determined by researcher.
Recommended Secondary Reagents
Goat Anti Rat IgG: HRP (mouse absorbed) F(ab')2 Goat anti-rat IgG: RPE (mouse adsorbed)
Fusion Partners
Spleen cells from an immunized DA rat were fused with cells of the Y3/Ag1.2.3 rat myeloma cell line.
Recommended Negative Controls
Rat IgG2a Negative Control
Storage and Stability
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Mouse Concanavilin A activated spleen cells. Epitope: Extracellular
Form
Supplied as a liquid, 0.09% sodium azide.
Purity
Purified by Protein G affinity chromatography.
Specificity
Mouse CD71 antigen, the transferrin receptor (TFR). Expressed on all dividing cells and can distinguish resting and activated T-cells. Immunoprecipitates a disulphide linked homodimer of 90kD by SDS PAGE. The antibody inhibits cell proliferation and the mixed lymphocyte response in vitro. Blocks the binding of R17 217.1.3. and R17 208.2 anti-TFR monoclonal antibodies (1,2).