Technical Data

T8199-30A
Clone Type
Monoclonal
Host
Rat
Source
Mouse
Isotype
IgG2a
Clone Number
3H3074 (YTA74.4)
Grade
Affinity Purified
Applications
FC IHC IP
Crossreactivity
Mo
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rat Anti-Transferrin Receptor (CD71, TR, Tfr, TfnrR)
Applications
Suitable for use in Flow Cytometry, Immunohistochemistry and Immunoprecipitation. Other applications have not been tested.
Recommended Dilution
Flow Cytometry: 1:50-1:100. 10ul to label 1x10e6 cells in 100ul. Immunohistochemistry: Frozen sections
Optimal dilutions to be determined by researcher.
Recommended Secondary Reagents
Goat Anti Rat IgG: HRP (mouse absorbed) F(ab')2 Goat anti-rat IgG: RPE (mouse adsorbed)
Fusion Partners
Spleen cells from an immunized DA rat were fused with cells of the Y3/Ag1.2.3 rat myeloma cell line.
Recommended Negative Controls
Rat IgG2a Negative Control
Storage and Stability
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Mouse Concanavilin A activated spleen cells. Epitope: Extracellular
Form
Supplied as a liquid, 0.09% sodium azide.
Purity
Purified by Protein G affinity chromatography.
Specificity
Mouse CD71 antigen, the transferrin receptor (TFR). Expressed on all dividing cells and can distinguish resting and activated T-cells. Immunoprecipitates a disulphide linked homodimer of 90kD by SDS PAGE. The antibody inhibits cell proliferation and the mixed lymphocyte response in vitro. Blocks the binding of R17 217.1.3. and R17 208.2 anti-TFR monoclonal antibodies (1,2).

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Lesley, J. et al. (1984). Expression of transferrin receptor on murine hematopoietic progenitors. Cellular Immunol. 83: 14-15.2. Trowbridge, I.S., et al. (1982). Murine cell surface transferrin receptor: studies with an a
USBio References
No references available
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