Mouse Anti-Transforming Growth Factor beta (TGFb)
Transforming growth factor-beta (TGF-beta) family of proteins has potent hematopoietic regulatory properties ranging from effects on the growth and differentiation of primitive stem cells to the differentiated functions of mature cells.
Applications
Suitable for use in Western Blot, ELISA, Neutralization and Immunohistochemistry. Other applications not tested.
Recommended Dilutions
Western Blot: 5-20ng/ml will allow visualization of 100ng/lane of TGF-beta. Recognizes the dimeric (25kD) and monomeric (12.5kD) forms of TGF-beta under both non-reducing and reducing conditions respectively. Neutralization: Neutralizes TGF-beta activity in vitro in an inhibition assay of CCL/64 cell growth and the growth promoting action of TGF-beta in the NRK-49F colony forming assay. The effect of microinjection of this antibody into one blastomere of two cell stage Xenopus embryos indicated that it was able to neutralize effectively the bioactivity of TGF-beta in vivo. Immunohistochemistry (FFPE/Frozen): 1:1000. Has been used to demonstrate TGF-beta in sheep ovarian tissue and human breast carcinoma. As a consequence of the intense staining of the erythrocytes, it is possible to locate a single cell within the ovarian stroma making it useful in locating very fine capillary networks within tissue. Optimal dilutions to be determined by the researcher.
Hybridoma
Sp2/0-Ag14 myeloma cells with spleen cells from Balb/c mice.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
TGF-b from human platelets.
Form
Supplied as a liquid in PBS, pH 7.0.
Purity
Purified by Protein G affinity chromatography.
Specificity
Recognizes both human platelet-derived and recombinant TGF-beta.
