Rabbit Anti-TRBP2 (Trans Activation Responsive RNA Binding Protein 2, Tar (HIV-1) RNA Binding Protein 2, TAR RNA-binding Protein 2, TARBP2, TRBP, LOQS, RISC-loading Complex Subunit TARBP2)
Trans-activation-responsive RNA-binding Protein
TRBP is an RNA associated nuclear protein. It contains 3 DRBM (double stranded RNA-binding) domains and plays an important role by binding to HIV-1 TAR RNA located in the long terminal repeat (LTR). TRBP acts synergistic with viral TAT protein in HIV-1 expression activation. It binds preferentially to TAR and other double-stranded RNAs with G+C-rich characteristics. It is important in regulation of transcription from RNA polymerase II promoter and is widely expressed in most of the tissues.
Applications
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilution
Western Blot: 5-8ug/ml Immunohistochemistry (paraffin): 5-10ug/ml Optimal dilutions to be determined by the researcher.
Positive Control
Human brain lysate
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to aa340-390 of human TRBP.
Form
Supplied as a liquid in PBS, 0.2% gelatin, 0.05% sodium azide.
Purity
Purified by Protein G affinity chromatography.
Specificity
Recognizes human TRBP. Species Crossreactivity: equine, monkey. Species sequence homology: mouse, rat, chicken.
